RP-modified silica plates (TLC and HPTLC)

Free choice of solvent systems for demanding separations and as a pilot technique for HPLC

Modified silica layers are well suited for many separation problems that cannot be adequately solved by unmodified silica. Advantages are:

• Results are less dependent on atmospheric humiditySeparation of extremely non-polar and highly polar substance using aqueous solvent systems
• RP-modified silica provides ready correlation with HPLC and allow TLC to be used as a pilot technique
• No catalytic activity towards non-stable substances that might tend to oxidative degradation

RP-modified silica plates
RP-2, RP-8 and RP-18 plates are based on silica gel 60 modified with aliphatic hydrocarbons. The chain length in combination with the degree of modification defines the ability to tolerate the water of the solvent system and strongly affects retention. Migration time increases in the order RP-2, RP-8, RP-18 using the same solvent composition. The HPTLC RP-2 sorbent exhibits higher polarity and a high affinity to aqueous solutions, tolerating up to 80% water, while the longer carbon chains RP-8 and R-18 can be run with up to 60% water in the solvent system. The specially developed HPTLC RP18W plate with a defined lower degree of surface modification can be used even with 100% water in the solvent system. The RP-18 silica plates with the concentrating zone are especially suited for the high-resolution separation of polycyclic aromatic hydrocarbons (PAH).

*Layer thickness: 200 μm | Layer thickness: 250 μm