DNA Transfection Reagent for insect cells
Insect GeneJuice® Transfection Reagent is a proprietary liposome formulation optimized for maximal transfection efficiency of insect cells including Sf9, S2, and HiFive® cells. The reagent features extremely low toxicity to the cells and can be used for both transient and stable transfections in serum-containing or serum-free media, and for cotransfection of transfer plasmids with linearized virus DNA for the production of recombinant baculovirus. Insect GeneJuice is ideal for large-scale protein expression in suspension-culture transfections of Sf9 insect cells when using pIEx™ and pBiEx™ vectors, which contain the immediate early baculovirus promoter, IE1.
Insect GeneJuice Transfection Reagentis provided as a 2 mg/ml suspension in 20 mM MES, 150 mM NaCl, pH 6.2 buffer. One milliliter is sufficient for 10 transfections in 10-ml suspension-culture flasks or 100 transfections in 35-mm plates.