BacMagic™ Systems

Using insect cells for recombinant protein expression is not a new process. However, this process has traditionally been tedious and labor intensive. In recent years there have been advancements in insect cell mediated expression making this a useful tool for recombinant protein expression. We continue to bring you new solutions for your insect expression needs.

BacMagic™ System

The original BacMagic™ System allows you to generate recombinant baculoviruses without the time-consuming plaque purification process. The next generation of the BacMagic System has the added advantage of improved quality and yield for most target proteins, through the deletion of additional non-essential genes.

BacMagic-2 DNA deletes chiA and v-cath genes, resulting in significantly improved quality and yield for most target proteins.

The BacMagic-3 DNA combines the deletion chiA and v-cath with the additional deletion of three more non-essential virus genes, p10, p74 and p26, for more efficient expression.

Faster baculovirus production without tedious plaque purification
Compatible with pIEx/Bac™, pBAC™, pTriEx™, and other transfer plasmids using the lef2/603 and ORF1629 recombination sites
Deletion of chitinase to maximize secreted and membrane-targeted protein production
Includes Insect GeneJuice^®Transfection Reagent for high efficiency transfection


Figure 1. Construction of baculovirus recombinants with three BacMagic systems

BacMagic™ System: No Plaque Purification Method

Day 1	Day 5	Days 8-10	Days 11-13
Cotransfect insect cells with recombinant transfer plasmid plus an AcNPV BacMagic™ DNA	Harvest recombinant baculovirus; screen for expression; amplify viral stock (titer stock, optional)	Infect insect cells and express protein	Harvest cells and proceed with purification

Ordering Information

	Artikelnummer	Produkt
[+]	72157	BacMagic™-2 DNA Kit

[+]	72158	BacMagic™-2 Transfection Kit

[+]	72350	BacMagic™-3 DNA Kit

[+]	71545	BacMagic™ DNA Kit

[+]	71546	BacMagic™ Transfection Kit