Using insect cells for recombinant protein expression is not a new process. However, this process has traditionally been tedious and labor intensive. In recent years there have been advancements in insect cell mediated expression making this a useful tool for recombinant protein expression. We continue to bring you new solutions for your insect expression needs.
The original BacMagic™ System allows you to generate recombinant baculoviruses without the time-consuming plaque purification process. The next generation of the BacMagic System has the added advantage of improved quality and yield for most target proteins, through the deletion of additional non-essential genes.
BacMagic-2 DNA deletes chiA and v-cath genes, resulting in significantly improved quality and yield for most target proteins.
The BacMagic-3 DNA combines the deletion chiA and v-cath with the additional deletion of three more non-essential virus genes, p10, p74 and p26, for more efficient expression.
|Figure 1. Construction of baculovirus recombinants with three BacMagic systems|
BacMagic™ System: No Plaque Purification Method
|Day 1||Day 5||Days 8-10||Days 11-13|
|Cotransfect insect cells with recombinant transfer plasmid plus an AcNPV BacMagic™ DNA||Harvest recombinant baculovirus; screen for expression; amplify viral stock (titer stock, optional)||Infect insect cells and express protein||Harvest cells and proceed with purification|
|[+]||72157||BacMagic™-2 DNA Kit|
|[+]||72158||BacMagic™-2 Transfection Kit|
|[+]||72350||BacMagic™-3 DNA Kit|
|[+]||71545||BacMagic™ DNA Kit|
|[+]||71546||BacMagic™ Transfection Kit|